Superscript first-strand synthesis system
WebSuperScriptŽ III First-Strand Synthesis System for RT-PCR Cat. No: 18080-051 Size: 50 reactions Store at -20°C Description The SuperScriptŽ III First-Strand Synthesis System for RT-PCR is optimized to synthesize first-strand cDNA from purified poly(A)+ or total RNA. RNA targets from 100 bp to >12 kb can be detected with this system. WebOct 1, 2006 · SuperScript First-Strand Synthesis System for RT-PCR (Invitrogen). 25 bp DNA ladder (Invitrogen). ... First, please include appropriate PCR controls to eliminate these possibilities. Some genes are expressed transiently or only in certain tissues. In our experience, this is the most likely cause for negative PCR results. Please read literature ...
Superscript first-strand synthesis system
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WebThe SuperScript® IV First-Strand Synthesis System for RT-PCR is optimized for synthesis of first-strand cDNA from purified poly(A)+ or total RNA. The kit includes SuperScript® IV … WebThe SuperScript™ III First-Strand Synthesis System for RT-PCR is optimized to synthesize first-strand cDNA from purified poly (A) + or total RNA. RNA targets from 100 bp to >12 kb can be detected with this …
WebSuperScriptŽ III First-Strand Synthesis System for RT-PCR Cat. No: 18080-051 Size: 50 reactions Store at -20°C Description The SuperScriptŽ III First-Strand Synthesis System … Web18091050 SuperScript™IV First-Strand Synthesis System Packaging Lot: 01102630 Expiry Date: 31.05.2024 (DD.MM.YYYY) Storage: at -20±5°C Filling lots for components in package: Lot Quantity Description 01077936 4 ku RNase Inhibitor 40 U/µL 01100208 10 ku SuperScript IV RT 200 U/µL 01101916 0.45 ku E.coli RNase H 2 u/µL 01075476 0.25 mL ...
WebFirst-strand cDNA synthesis for thermostability Reverse transcription was performed as described above using oligo(dT) 20 and 500 ng Millennium RNA Markers, with the exception of reaction temperature that ranged from 50 to 65°C. WebBased on our findings, the kit with the high- final volume for all reactions was adjusted to 50 μl with est acquired score was the SuperScript® III Reverse Tran- ddH2O. cDNA was stored at - 30°C overnight and then scriptase, by Invitrogen, achieving an overall score of 18, used for RQ- PCR. followed by the First Strand cDNA Synthesis Kit for ...
WebThe SuperScript® III First-Strand Synthesis System for RT-PCR is used to synthesize first-strand cDNA from purified total RNA. RNA targets from 100 bp to >12 kb can be dete...
WebThe SuperScript® IV First-Strand Synthesis System for RT-PCR is optimized for synthesis of first-strand cDNA from purified poly (A)+ or total RNA. The kit includes SuperScript® IV Reverse Transcriptase (RT), a proprietary MMLV mutant that produces robust and reliable cDNA synthesis in RT reactions. dahl first dimension powerWebThe first-strand cDNA synthesis reaction is catalyzed by SuperScript ™ II Reverse Transcriptase (RT). This enzyme has been engineered to This enzyme has been … bio cybill shepherdWebFeatures of the SuperScript IV First-Strand Synthesis System include: Significantly improved resistance to a variety of inhibitors that can interfere with cDNA synthesis … dahl ford body shopWebSuperScript LV Reverse Transcriptase—the following generation reverse transcriptase is field to have better efficiency, sensitivity, inhibitor tolerance, reaction time, and thermostability compared to its predecessors. ... GeneArt General Synthesis ... dahl ford davenport phone numberWebSystem • Optimized reagents and protocol ensure highly robust and reproducible labeling reactions • SuperScript™ III Reverse Transcriptase in the first-strand synthesis reaction produces high yields of cDNA, greater incorporation of fluorescent nucleotides, and higher signal-to-background ratios with small amounts of starting material biocycle hamiltonWebThe SuperScript™ IV First-Strand Synthesis System for RT-PCR is optimized for synthesis of first-strand cDNA from purified poly(A)+ or total RNA. The kit includes SuperScript™ IV … The SuperScript™ IV First-Strand Synthesis System for RT-PCR is optimized for … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes biocycle east coast conferenceWebMutations in the RNase H domain of the enzyme eliminate degradation of the RNA during first-strand cDNA synthesis, which results in higher yields of full-length cDNA. SuperScript RTs are the most highly trusted and widely used RTs with over 50,000 citations, reviews, and publications to date. Invitrogen™ SuperScript™ II Reverse Transcriptase dahl fine arts center rapid city