Itgax cre小鼠
Web4 mrt. 2024 · 其次为 Cre鼠和flox鼠。 Cre为一种酶,因其具有位点特异性,可以使Loxp片段间的基因序列被删除或重组。 因此,Cre与Loxp一起组成Cre-Loxp系统,从而实现在时 … WebCre鼠常用于与Flox小鼠杂交进行条件性的目的基因敲除,但也有研究将Cre鼠用于切割位于报告基因前的终止序列,以达到荧光标记特定细胞的目的,进一步研究细胞命运。
Itgax cre小鼠
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Web如果有多种不同的 Cre,那么就需要分别表示。 例如:Tlr5 flox 小鼠分别与小肠上皮细胞(IEC)特异性 Cre(Vil-Cre)、DC 细胞特异性 Cre(CD11c-Cre) 交配的子代中发生基因 … Web022071 B6.Cg-Tg(Cd4-cre)1Cwi/BfluJ CD4-Cre transgenic mice contain CD4 enhancer, promoter and silencer sequences driving the expression of a Cre recombinase gene. These mice may be useful for generating conditional mutations in CD4-expressing tissues.
WebItgax-Cre小鼠. 该Cre小鼠与FloxP小鼠交配后,在Cre特异表达的组织或者器官或细胞中,两个FloxP位点间的外显子或元件将被删除,达到条件性敲除或过表达的目的。. Cre-loxP … Web28 mrt. 2024 · Mutation details : For generation of CD11c.DTR BAC transgenic mice, a fusion construct composed of the DNAs for the human DTR, ovalbumin fragment containing sequences encoding amino acids 140-386, and eGFP was generated and inserted at the start codon of the CD11c gene in the BAC RPCI-24-7812. The eGFP component of the …
WebThese Cd11c-Cre transgenic mice are a Cre-lox tool for deletion of floxed sequences in CD8 - , CD8 + dendritic cells, tissue-derived dendritic cells from lymph nodes, lung and … WebBAC clone RP24-361C4 (BACPAC Resources) containing the Itgax (Cd11c) gene but lacking the 5' end of the adjacent Itgam gene, was modified by ET recombination to generated the transgene construct. Founder line 1-1 was consequently established. Cre recombinase expression is detected in CD8-, CD8+ dendritic cells, tissue derived …
Web007567 C57BL/6J-Tg(Itgax-cre,-EGFP)4097Ach/J These CD11c-Cre-GFP transgenic mice may be useful in immunological studies utilizing Cre-lox technology or fluorescent protein expression in dendritic cells.
Web004781 B6.129P2- Lyz2 tm1(cre)Ifo /J The LysMcre knock-in/knock-out allele has a nuclear-localized Cre recombinase inserted into the first coding ATG of the lysozyme 2 gene ( Lyz2 ); both abolishing endogenous Lyz2 gene function and placing NLS-Cre expression under the control of the endogenous Lyz2 promoter/enhancer elements. education needed for investment bankingWebAarts SA等人通过在CD40基因2~3号外显子之间插入loxP-Neo-loxP元件破坏CD40基因,通过与LysM cre 小鼠杂交产生骨髓特异性CD40敲除小鼠 [4] 。 研究发现,CD40 fl/fl LysM … education needed for human resourceseducation needed for nfl playerWeb7 aug. 2024 · 2.1 新的Cre品系需要做的四件事:. 1) 了解小鼠来源,背景信息及发表的文章;. 2) 建立基因型鉴定方法,确认小鼠正确;. 3) 验证Cre在目标组织中的表达. 4) 验证Cre的敲除效率. 了解小鼠来源,背景信息及发表的文章. Mouse Genome Informatics CrePortal :. 在Recombinase (cre)中 ... education needed for obstetricianWebCre-ER 系统,特别是 Cre-ERT2 ,是目前应用最广泛的诱导型 Cre 系统。其较之四环素诱导型 Cre,系统更为简单。我们只需要将 Cre-ERT2 设计在组织特异性启动子之后,并与 … construction skills city and guildsWebCre工具鼠是通过在小鼠marker基因位点敲入Cre/CreERT2或者Dre/DreERT2元件构建而成。 Cre工具鼠跟特定的flox小鼠交配,可以实现在巨噬细胞中敲除或者敲入目的基因,完成 … construction skills citbWeb基因工程鼠目录. 工具基因工程小鼠(胚胎冷冻或精子冷冻) 1 系统表达绿色荧光转基因小鼠(Chikenβ-actin-eGFP) 2 系统表达荧光素酶转基因小鼠(Chikenβ-actin-Luc) 3 脑组织 … construction skills clayton