Hirudin binding kd
Web16 nov 2024 · The hirudin-like factor 1 (HLF1) of Hirudo medicinalis belongs to a new class of leech-derived factors. In previous investigations, HLF1 did not exhibit anticoagulatory activities. Here, we describe the analysis of natural and synthetic variants of HLF1 and HLF-Hyb, a yet uncharacterized member of the HLF family. WebAn anticoagulant hirudin was produced using oleosin as a carrier (Parmenter et al., 1995; van Rooijen and Moloney, 1995; Chaudhary et al., 1998 ). Oleosins are a class of seed …
Hirudin binding kd
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Web1 ago 1990 · The carboxyl tail of hirudin (residues 48-65) wraps around thrombin along the putative fibrinogen secondary binding site. This long groove extends from the active site cleft and is flanked by the thrombin loops 35-39 and 70-80. Hirudin makes a number of ionic and hydrophobic interactions with thrombin in this area. WebThe X-ray crystallographic structure of the hirudin–thrombin complex coupled with earlier structure–function studies indicate that the amino-terminal domain of hirudin interacts with the active site of thrombin’s catalytic triad residue-serine 195 and its carboxy-terminal domain binds to the positively charged anion-binding exosite I (Rydel et al., 1990, 1991; …
Web15 gen 1990 · The experiments were performed (a) to examine the impor- tance of the amino acid residue adjacent to the putative reactive site, and (b) to dissect hirudin inhibitory activity and 713 This is an Open Access article under the CC BY license. 714 Binding Sites of Ala48-Hirudin-derived Fragments on wThrombin to localize binding sites of the …
Mouse monoclonal Hirudin antibody [2D7]. Validated in WB, ELISA and tested in Human. ... (Kd) of this antibody for rHV2K47 is 4.5x10E-7 M. Tested applications. Suitable for: ELISA, ... We test ab17198 and ab36390 byaccessing their binding abilities to human thrombin and to complex of human thrombin with the thromobin inhibitor hirudin. Web25 giu 1993 · 4HTC. PubMed Abstract: The structure of a recombinant hirudin (variant 2, Lys47) human alpha-thrombin complex has been refined using restrained least-squares …
Web10 ott 2024 · The kinetic constants including the association constant (k a), k d (dissociation constant), and equilibrium constants (KD) were calculated according to 1:1 binding …
WebHirudin derivatives (e.g. lepirudin, desirudin) and hirudin analogues (e.g. bivalirudin) are bivalent direct thrombin inhibitors; that is, they bind to two distinct sites on thrombin-its active (catalytic) site and its fibrinogen-binding site (exosite 1). These bivalent binding properties contribute to their high affinity and high specificity ... methodist university hospital molliWeb10 mar 1990 · Only the monoclonal antibody 4049-83-12, obtained from the group I of mice, showed high affinity for hirudin (Kd of 0.6 nM) and in vitro neutralizing properties. The … methodist university hospital addressWebBraun PJ (1990) Binding properties of hirudin determined by gel filtration and gel electrophoresis. Thromb Res 59: 657–662. Google Scholar Brüggener E, Walsmann P, … methodist university hospital memphisWeb1 giu 1998 · Hirudin is a 6.97 kd protein that specifically and irreversibly binds to both the catalytic and fibrinogen binding sites of activated thrombin. 16, 17 Although the clinical use of hirudin systemically has been limited because of an increased potential for spontaneous hemorrhage, 18, 19 animal experiments indicate that the use of hirudin via ... how to add integers in pythonWeb15 set 1991 · A thrombin receptor has recently been cloned and the sequence deduced. The sequence reveals a thrombin cleavage site that accounts for receptor activation. The … methodist university hospital remote loginWeb20 dic 2014 · The anti-thrombin activity of the six mutants and RGD-hirudin was tested. Further, we evaluated the binding of the mutant variants and RGD-hirudin to thrombin … methodist university human resourcesWeb27 nov 1990 · Equilibrium binding of thrombin to recombinant human thrombomodulin: effect of hirudin, fibrinogen, factor Va, and peptide analogues Biochemistry . 1990 Nov … methodist university hospital phone number