WebD. The x-intercept in a Lineweaver-Burk plot corresponds to -1/Km, and the y-intercept corresponds to 1/Vmax. Therefore, we can use the values of Vmax and Km obtained from the graph to calculate the numerical values of the x- and y-intercepts. The equation for a Lineweaver-Burk plot is: 1/V0 = (Km/Vmax) * (1/[A]) + 1/Vmax WebPractice: To determine the V max from a Lineweaver-Burk plot you would: Multiply the reciprocal of the x-axis intercept by -1. Multiply the reciprocal of the y-axis intercept by -1. Take the reciprocal of the x-axis intercept. …
4.10: Lineweaver-Burk Plots - Biology LibreTexts
WebSep 1, 2024 · Another commonly-used plot in examining enzyme kinetics is the Lineweaver-Burk plot, in with to inverse of the feedback rate, \(1/r\), is plotted against the inverse of the substrate concentration \(1/\left[ \text{S} \right]\). Rearranging Equation \(\ref{Eq13.26}\), ... and using the slopes ours find that K m is. K m = slope × V max = … WebThe reciprocal of this equation is: 1 / V0 = (KM / Vmax [S]) + (1 / Vmax) From this, the Lineweaver Burk equation becomes: 1 / V0 = (KM / Vmax ) (1 / [S]) + (1 / Vmax) One thing to note about the Lineweaver-Burk … city of henry illinois
Lineweaver-Burk plot of AChE for parietin. - ResearchGate
WebThe Michaelis-Menten equation is used to find the kinetic constants of a reaction, such as the maximum rate (Vmax) and the Michaelis constant. The Lineweaver Burke plot is a double-reciprocal plot of the Michaelis-Menten equation. It is used to determine these constants. (Km). WebApr 9, 2024 · The Lineweaver-Burk plot is used to determine Vmax and Km. There is a Lineweaver-Burk plot of the data. The Km and Vmax are represented in a Lineweaver … WebApr 14, 2024 · In this lecture, we explain the double reciprocal plot, a graphical method commonly used for the accurate determination of the Km and Vmax of enzymes. city of hephzibah city hall