Weband EIF2S1 phosphorylation could also selectively inhibit the translation of proteins prone to aggregation, which has been attributed to induction of RIDD.23 Furthermore, accumulation of proteins on the ER membrane leads to activation of the tran-scription factor NFKB, which initially stimulates the expression WebJun 19, 2024 · Different intrinsic and extrinsic stress pathways including endoplasmic reticulum (ER) stress converge on the phosphorylation of eukaryotic translation initiation …

EIF2α phosphorylation: a hallmark of both autophagy and ... - PubMed

WebPaclitaxel-mediated cell death was significantly increased following loss of ISR or ATF4 expression. This sensitizing effect could be partially rescued by Trolox, a ROS scavenger. We demonstrated that the alternative initiation factor EIF2A was essential for cancer cell survival after paclitaxel-mediated ISR both in vitro and in vivo. WebJun 19, 2024 · Different intrinsic and extrinsic stress pathways including endoplasmic reticulum (ER) stress converge on the phosphorylation of eukaryotic translation initiation factor 2A (EIF2A, best known as eIF2α), which characterizes the so-called "integrated stress response". This phosphorylation event is imp … google input tools filehippo

EIF2S1 Gene - GeneCards IF2A Protein IF2A Antibody

WebImmunocytochemistry analysis of Phospho-EIF2S1 (Ser51, Ser52) in HepG2 cells (4hrs of LPS treatment). Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody ( Product # PA5-114570) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied ... WebJan 23, 2007 · EIF2S1/eIF-2-alpha is a key component of the integrated stress response (ISR), required for adaptation to various stress: phosphorylation by metabolic-stress … WebPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminal sequence of eIF2α. Antibodies are purified by … chic bourdoir handbags spring 2016