Ctcf shrna
WebJul 20, 2024 · Using shRNA targeting CTCF to recapitulate CTCF haploinsufficiency, we have identified a novel role for CTCF in the regulation of cellular polarity of endometrial glandular epithelium. Overall, we have identified two novel pro-tumorigenic roles (promoting cell survival and altering cell polarity) for genetic alterations of CTCF in endometrial ... WebGenetic locus: CTCF (human) mapping to 16q22.1. PRODUCT CTCF siRNA (h) is a pool of 3 target-specific 19-25 nt siRNAs designed to knock down gene expression. Each vial contains 3.3 nmol of lyophilized siRNA, sufficient for a 10 µM solution once resuspended using protocol below. Suitable for 50-100 transfections. Also see CTCF shRNA Plasmid …
Ctcf shrna
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WebSep 4, 2024 · (A) Bar chart showing the number of CTCF loops with different loop strength in control shRNA and BHLHE40 shRNA-depleted HeLa-S3 cells. ( B ) Boxplot showing … WebThe site navigation utilizes arrow, enter, escape, and space bar key commands. Left and right arrows move across top level links and expand / close menus in sub levels.
WebNov 5, 2012 · Mutation of CTCF-binding sites in plasmid-borne promoters reduced transcriptional activity in an orientation-dependent manner. Depletion of CTCF by … WebNov 30, 2024 · CCCTC-binding factor (CTCF) is a conserved transcription factor that performs diverse roles in transcriptional regulation and chromatin architecture. ...
WebDec 26, 2024 · Results: We knocked down CTCF in EL4 cells using shRNA, and conducted single cell RNA-seq on both wild type (WT) cells and CTCF-Knockdown (CTCF-KD) cells using Fluidigm C1 system. Principal component analysis of single cell RNA-seq data showed that WT and CTCF-KD cells concentrated in two different clusters on PC1, … WebNSD2 Low cell lines were generated in these three B-ALL cell lines because they naturally harbor a heterozygous NSD2 EK mutation. More specifically, a short-hairpin RNA (shRNA: GGAAACTACTCTCGATTTATG) was used to knockdown NSD2 targeting the NSD2 Type II and NSD2 RE-IIBP isoforms both of which contain the SET domain.
WebSep 1, 2016 · In CTCF-shRNA cells, CTCF expression was further diminished and PAX6, ADAM17 and RCN1 protein expressions were enhanced following the time course. Effects of altered CTCF activity on PAX6 and PAX6-associated ADAM17 and RCN1 expression were further measured by RT-PCR in both Lv-control and CTCF-shRNA HTCE cells.
WebAug 23, 2013 · Lentiviral particles containing shRNA of CTCF tagged with a variant of green fluorescent protein (Turbo-GFP, Sigma-Aldrich, St. Louis, MO) were packaged in HEK-293T cells (17). The viral concentrations in the culture medium were titrated by PCR after cotransfection of HTCE cells with pCMV-VSV-G, psPAX2, and pGIPZshRNA-CTCF … arkansas baseball starting lineup 2023WebCTCF (the CCCTC-binding factor) is a sequence-specific DNA binding protein that recognizes extended DNA motifs with its 11 zinc fingers and acts as a critical regulator of interphase genome folding (Splinter et al ... (NT) shRNA. (G) Effects of 2 h of LPS stimulation on the 7543 H3K9me3 blocks methylated by SETDB1. (H) ... arkansas baseball stadiumWebNov 30, 2024 · Inhibition of cell proliferation and clonogenicity following CTCF shRNA knockdown in K562 cells. (A) Immunoblot of CTCF shRNA knockdown in the presence and absence of doxycycline (dox) compared to ... balirando jassyWebSep 1, 2016 · Our results explain how epigenetic factor CTCF-mediated chromatin remodeling regulates interactions between eye-specific PAX6 and those genes that are induced/associated with cell differentiation to modulate corneal epithelial cell-specific differentiation. ... Diminished CTCF mRNA with CTCF-shRNA in HTCE cells weakened … balirando iti 0589WebNov 30, 2024 · 2. Results. We used shRNA knockdown to model the cellular consequences of reduced CTCF expression in K562 cells. Western blots showed that CTCF protein expression was significantly knocked down by ~80% in the presence of doxycycline (dox) in sh.CTCF K562 cells compared to non dox-treated cells and sh.control cells (Figure 1 … arkansas baseball rpiWebNational Center for Biotechnology Information arkansas baseball stadium mapWebThe site navigation utilizes arrow, enter, escape, and space bar key commands. Left and right arrows move across top level links and expand / close menus in sub levels. arkansas baseball shirts