WebGreen fluorescence protein (GFP) is a reporter protein of 238 amino acids, and it emits a bright green fluorescence (λ max = 509 nm) when illuminated with a blue light (λ max = … WebDAPI GFP RFP Texas Red Cy5 Additional filters can be purchased by labs and used on the EVOS, if desired. IF YOU ARE TRYING TO IMAGE GFP AND “RED”, YOU MUST USE THE TEXAS RED FILTER INSTEAD OF THE RFP FILTER. GFP BLEEDS INTO THE RFP FILTER TO THE POINT THAT YOU CANNOT DISTINGUISH BETWEEN THE TWO. …
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WebThe DAPI-FITC-TRITC triple band fluorescence filter combination is designed specifically for simultaneous detection of the fluorochromes DAPI, FITC, and TRITC with minimal crossover (spectral bleed-through) between adjacent bands, and it can also be employed with other combinations of fluorescent probes that have similar spectral profiles.The … WebThe BioTek Cytation 5 cell imaging multimode reader combines automated digital widefield microscopy with conventional multimode microplate reading. Fluorescence, brightfield, color brightfield, and phase contrast imaging are available with the automated cell imager. Fluorescence, luminescence, and UV-Vis absorbance multimode plate reading … pdf online unlock free
Microscopy UCSF Helen Diller Family …
WebCytation 5 automates many traditionally manual microscopy tasks, from slide scanning to time-lapse live cell assays; from low to high magnification. Cytation 5 is ready for any imaging assay. Flexible hardware has 6-objective turret, 1.25x to 60x, 20+ colors available and a wide FOV camera. WebThe system is setup to acquire up to 4 fluorescent channels plus transmitted light. The four filter sets available are for fluorochromes with excitation/emission spectrum comparable to DAPI/Hoescht/Coumarin, GFP/FITC/Cy2/Al488, Rhodamine/Texas Red/Cy3/mRFP, Cy5. We anticipate an hourly rate of $15/hr for use. Training will be free. WebCy5 Filter Set. This Iridian single band filter set consists of the 628-40 EX, 692-40 EM and 660 DM filters. It is optimised for the cyanine dye Cy5. The set contains excitation, emission and dichroic filters designed to fit in … pdf online unlocker